Protein Structure Determination

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Concept

We provide contract studies on protein structure determinations and other experiments related to the protein structure and interactions. We can express, label by stable heavy isotopes and purify the protein of your interest and finally, we are able to determine its structure by NMR spectroscopy methods. The service is provided on commercial basis. Please contact nmr-sales@asla-biotech.com for details.

 

Equipment

We have a permanent access to 4 chanel ( 1H, 13C, 15N, 2D) NMR spectrometer Varian INOVA 600 MHz equipped with cryoprobe located at the Latvian Institute of Organic Synthesis

NMR expertize

Dr Edvards Liepinsh, OSI, NMR of proteins and nucleic acids

 

 

Publications

Liepinsh E, Rakonjac M, Boissonneault V, Provost P, Samuelsson B, Radmark O, Otting G. NMR structure of human coactosin-like protein. J Biomol NMR. 2004 Nov;30(3):353-6. No abstract available. PMID: 15754059 [PubMed - indexed for MEDLINE]

Williams NK, Liepinsh E, Watt SJ, Prosselkov P, Matthews JM, Attard P, Beck JL, Dixon NE, Otting G. Stabilization of native protein fold by intein-mediated covalent cyclization. J Mol Biol. 2005 Mar 4;346(4):1095-108. Epub 2005 Jan 18. PMID: 15701520 [PubMed - indexed for MEDLINE]

Oakley AJ, Loscha KV, Schaeffer PM, Liepinsh E, Pintacuda G, Wilce MC, Otting G, Dixon NE. Crystal and solution structures of the helicase-binding domain of Escherichia coli primase. J Biol Chem. 2005 Mar 25;280(12):11495-504. Epub 2005 Jan 12. PMID: 15649896 [PubMed - indexed for MEDLINE]

Moriya M, Taniguchi S, Wu P, Liepinsh E, Otting G, Sagara J. Role of charged and hydrophobic residues in the oligomerization of the PYRIN domain of ASC. Biochemistry. 2005 Jan 18;44(2):575-83. PMID: 15641782 [PubMed - indexed for MEDLINE]

Hosia W, Bark N, Liepinsh E, Tjernberg A, Persson B, Hallen D, Thyberg J, Johansson J, Tjernberg L. Folding into a beta-hairpin can prevent amyloid fibril formation. Biochemistry. 2004 Apr 27;43(16):4655-61. PMID: 15096033 [PubMed - indexed for MEDLINE]

Modig K, Liepinsh E, Otting G, Halle B. Dynamics of protein and peptide hydration. J Am Chem Soc. 2004 Jan 14;126(1):102-14. PMID: 14709075 [PubMed - indexed for MEDLINE]

Liepinsh E, Barbals R, Dahl E, Sharipo A, Staub E, Otting G. The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition. J Mol Biol. 2003 Oct 3;332(5):1155-63. PMID: 14499617 [PubMed - indexed for MEDLINE]

Liepinsh E, Banyai L, Pintacuda G, Trexler M, Patthy L, Otting G. NMR structure of the netrin-like domain (NTR) of human type I procollagen C-proteinase enhancer defines structural consensus of NTR domains and assesses potential proteinase inhibitory activity and ligand binding. J Biol Chem. 2003 Jul 11;278(28):25982-9. Epub 2003 Apr 1. PMID: 12670942 [PubMed - indexed for MEDLINE]

Liepinsh E, Genereux C, Dehareng D, Joris B, Otting G. NMR structure of Citrobacter freundii AmpD, comparison with bacteriophage T7 lysozyme and homology with PGRP domains. J Mol Biol. 2003 Apr 4;327(4):833-42. PMID: 12654266 [PubMed - indexed for MEDLINE]

Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G. Solution structure of the R3H domain from human Smubp-2. J Mol Biol. 2003 Feb 7;326(1):217-23. PMID: 12547203 [PubMed - indexed for MEDLINE]

Liepinsh E, Otting G, Harding MM, Ward LG, Mackay JP, Haymet AD. Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein. Eur J Biochem. 2002 Feb;269(4):1259-66. PMID: 11856360 [PubMed - indexed for MEDLINE]

Williams NK, Prosselkov P, Liepinsh E, Line I, Sharipo A, Littler DR, Curmi PM, Otting G, Dixon NE. In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem. 2002 Mar 8;277(10):7790-8. Epub 2001 Dec 12. PMID: 11742000 [PubMed - indexed for MEDLINE]

Liepinsh E, Trexler M, Kaikkonen A, Weigelt J, Banyai L, Patthy L, Otting G. NMR structure of the LCCL domain and implications for DFNA9 deafness disorder. EMBO J. 2001 Oct 1;20(19):5347-53. PMID: 11574466 [PubMed - indexed for MEDLINE]

Liepinsh E, Baryshev M, Sharipo A, Ingelman-Sundberg M, Otting G, Mkrtchian S. Thioredoxin fold as homodimerization module in the putative chaperone ERp29: NMR structures of the domains and experimental model of the 51 kDa dimer. Structure (Camb). 2001 Jun;9(6):457-71. PMID: 11435111 [PubMed - indexed for MEDLINE]

Yang Y, Griffiths WJ, Nordling M, Nygren J, Moller L, Bergman J, Liepinsh E, Otting G, Gustafsson JA, Rafter J, Sjovall J. Ring opening of benzo[a]pyrene in the germ-free rat is a novel pathway for formation of potentially genotoxic metabolites. Biochemistry. 2000 Dec 19;39(50):15585-91. PMID: 11112546 [PubMed - indexed for MEDLINE]

Henriksson M, Shafqat J, Liepinsh E, Tally M, Wahren J, Jornvall H, Johansson J. Unordered structured of proinsulin C-peptide in aqueous solution and in the presence of lipid vesicles. Cell Mol Life Sci. 2000 Feb;57(2):337-42. PMID: 10766028 [PubMed - indexed for MEDLINE]

Liepinsh E, Sodano P, Tassin S, Marion D, Vovelle F, Otting G. Solvation study of the non-specific lipid transfer protein from wheat by intermolecular NOEs with water and small organic molecules. J Biomol NMR. 1999 Nov;15(3):213-25. PMID: 10677825 [PubMed - indexed for MEDLINE]

Ilag LL, Rottenberger C, Liepinsh E, Wellnhofer G, Rudert F, Otting G, Ilag LL. Selection of a peptide ligand to the p75 neurotrophin receptor death domain and determination of its binding sites by NMR. Biochem Biophys Res Commun. 1999 Feb 5;255(1):104-9. PMID: 10082663 [PubMed - indexed for MEDLINE]

Leonchiks A, Liepinsh E, Barishev M, Sharipo A, Masucci MG, Otting G. Random coil conformation of a Gly/Ala-rich insert in IkappaB alpha excludes structural stabilization as the mechanism for protection against proteasomal degradation. FEBS Lett. 1998 Dec 4;440(3):365-9. PMID: 9872404 [PubMed - indexed for MEDLINE]

Andersson P, Nordstrand K, Sunnerhagen M, Liepinsh E, Turovskis I, Otting G. Heteronuclear correlation experiments for the determination of one-bond coupling constants. J Biomol NMR. 1998 May;11(4):445-50. PMID: 9691285 [PubMed - indexed for MEDLINE]

Hamberg M, Liepinsh E, Otting G, Griffiths W. Isolation and structure of a new galactolipid from oat seeds. Lipids. 1998 Apr;33(4):355-63. PMID: 9590622 [PubMed - indexed for MEDLINE]

Ruysschaert JM, Goormaghtigh E, Homble F, Andersson M, Liepinsh E, Otting G. Lipid membrane binding of NK-lysin. FEBS Lett. 1998 Mar 27;425(2):341-4. PMID: 9559676 [PubMed - indexed for MEDLINE]

Liepinsh E, Kitamura M, Murakami T, Nakaya T, Otting G. Common ancestor of serine proteases and flavin-binding domains. Nat Struct Biol. 1998 Feb;5(2):102-3. No abstract available. PMID: 9461072 [PubMed - indexed for MEDLINE]

Liepinsh E, Kitamura M, Murakami T, Nakaya T, Otting G. Pathway of chymotrypsin evolution suggested by the structure of the FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F) Nat Struct Biol. 1997 Dec;4(12):975-9. No abstract available. PMID: 9406543 [PubMed - indexed for MEDLINE]

Liepinsh E, Andersson M, Ruysschaert JM, Otting G. Saposin fold revealed by the NMR structure of NK-lysin. Nat Struct Biol. 1997 Oct;4(10):793-5. PMID: 9334742 [PubMed - indexed for MEDLINE]

Liepinsh E, Ilag LL, Otting G, Ibanez CF. NMR structure of the death domain of the p75 neurotrophin receptor. EMBO J. 1997 Aug 15;16(16):4999-5005. PMID: 9305641 [PubMed - indexed for MEDLINE]

Sorensen MD, Bjorn S, Norris K, Olsen O, Petersen L, James TL, Led JJ. Solution structure and backbone dynamics of the human alpha3-chain type VI collagen C-terminal Kunitz domain,. Biochemistry. 1997 Aug 26;36(34):10439-50. PMID: 9265624 [PubMed - indexed for MEDLINE]

Otting G, Liepinsh E, Halle B, Frey U. NMR identification of hydrophobic cavities with low water occupancies in protein structures using small gas molecules. Nat Struct Biol. 1997 May;4(5):396-404. PMID: 9145111 [PubMed - indexed for MEDLINE]